Laboratory of Plant Molecular Biology and Virology (LPMBV)

LPMBV has recently been founded to seek a molecular understanding of various phenomena in plant and virus resources, including 1) gene (RNA) silencing using transgenic plants, 2) microarray analysis on plant defense reaction against pathogens and 3) analysis of plant viral genes and interaction between plant mRNA and viral RNA. Through these studies we hope to contribute to the development of new plant resources and to increase the human food supply in the world.

Key words

Transformed plants, Gene transfer, Gene expression, Gene silencing, DNA, RNA, Cloning, Sequencing, Plant virus, Plant defense, Protoplasts

Academic Staff of the Laboratory

Professor/ Dr. Masamichi NISHIGUCHI

email ：mnishiguATagr.ehime-u.ac.jp (use @ instead AT)

TEL&FAX:+81-89-946-9816 FAX:+81-89-977-4364(Adm. Office)

Recent Publications

• 1)Haque, Y.,Tanaka, S.Sonoda.M.,Nishiguchi,M.(2006) Analysis of transitive RNA silencing after grafting in transgenic plants with the coat protein gene of Sweet potato feathery mottle virus. Plant Mol. Biol. (in press)

• 2) M. Nishiguchi, S. Yamasaki, X.-Z. Lu, A. Shimoyama, K. Hanada, S. Sonoda, M. Shimono, J. Sakai, Y. Mikoshiba, and I. Fujisawa (2006) Konjak mosaic virus: the complete nucleotide sequence of the genomic RNA　and its comparison with other potyviruses. Arch. Virol.151:1643-1650.

• 3) M. Asano, R. Satoh, A. Mochizuki, S. Tsuda, T. Yamanaka, M. Nishiguchi, K. Hirai, T. Meshi, S. Naito, M. Ishikawa (2005) Tobamovirus-resistant tobacco generated by RNA interference directed against host genes. FEBS Lett. 579(20):4479-4484.

• 4) M. Shimono, M. Ino, S. Sonoda, T. Fujimura and M. Nishiguchi (2005) Inverse-corelation between the Virus resistance in Nicotiana benthamiana plants showing different levels of GFP gene silencing. J. Gen. Plant Pathol. 71: 147-152.

• 5) S. Sonoda, M. Nishiguchi and H. Tsumuki (2005) Evaluation of virus resistance conferred by the NS gene sequences from Tomato spotted wilt virus in transgneic plants. Breeding Sci. 55: 27-33.

• 6) Y. Matsushita, M. Ohshima, K. Yoshioka, M. Nishiguchi and H. Nyunoya (2003). The catalytic subunit of protein kinase CK2 phosphorylates in vitro the movement protein of Tomato mosaic virus. J Gen Virol 2003;84: 497-505

• 7) M. Shimono, J. Yazaki, K. Nakamura, N. Kishimot, S. Kikuchi,M. Iwano, K. Yamamoto,T. Sasaki, and M. Nishiguchi (2003). cDNA microarray analysis of gene expression in rice plants treated with probenazole, a chemical inducer of disease resistance.　J. Gen. Plant Pathol. 69: 76-82.

• 8) Y. Okada, A. Saito, M. Nishiguchi, T. Kimura, M. Mori, Y. Matsuda and T. Murata(2002). Microprojectile bonbardment-mediated transformation of sweet potato (Ipomoea batatas (L.) Lam). SABRAO J. Breed. Genet. 34(1), 1-8.

• 9) Y. Okada, M. Nishiguchi, A. Saito, T. Kimura, M. Mori, K. Hanada, J. Sakai, Y. Matsuda and T. Murata(2002). Inheritance and stability of the virus-resistance gene in the progeny of　transgenic sweet potato. Plant Breed. 121, 249-253.

• 10) Y. Matsushita, O. Miyakawa, M. Deguchi, M., M. Nishiguchi1 and H. Nyunoya (2002). Cloning of a tobacco cDNA coding for a putative transcriptional coactivator MBF1 that interacts with the tomoto mosaic virus movemnet protein. J. Expt. Bot. 53:1531-1532.

• 11) Y. Okada, A. Saito, M. Nishiguchi, T. Kimura, M. Mori, K. Hanada, J. Sakai, C. Miyazaki, Y. Matsuda and T. Murata (2001). Virus resistance in transgenic sweet potato (Ipomoea batatas L.(Lam)) expressing the coat protein gene of sweet potato feathery mottle virus. Theor. Appl. Genet. 103: 743-751.

• 12) R. Nagata, M. Mori, K. Hanada and M. Nishiguchi(2001). An improved methodof reverse transcription and polymarase reaction (RT-PCR) to efficiently detect potyvirus, sweet potato feathery mottle virus (SPFMV) RNA in sweet potato. J. Gen. Plant Pathol. 67:164-168.

• 13) Y. Matsushita, M. Deguchi, M. Youda, M. Nishiguchi and H. Nyunoya(2001). The tomato mosaic tobamovirus movement protein interacts with a putative transcriptional coactivator KELP. Mol. Cells 12: 57-66.

• 14) N. Kubo, M. Takano, M. Nishiguchi and K.Kadowaki (2001). Mitochondrial sequence migrated downstream to a nuclear V-ATPase B gene is transcribed, but not functional. Gene 271;193-201.

• 15) S. Sonoda and M. Nishiguchi (2000). Graft-transmission of posttranscriptional gene silencing in transgenic plants; target specificity for RNA degradation is transmissible between silenced and non-silenced plants, but not between silenced plants. Plant J. 21(1):1-8.

• 16) S. Sonoda, M. Kanda, H. Koiwa, H. Kato and M. Nishiguchi (2000). The helper component-proteinase of sweet potato feathery mottle potyvirus facilitates systemic spread of unlelated viruses in Ipomoea nil. 　Phytopathology 90:944-950.

• 17) T. Sian-Hee, M. Nishiguchi, M. Murata and F. Motoyoshi (2000). The genome structure of kyuri green mottle mosaic virus in comparison with cucumber green mottle mosai virus. Arch. Virol. 145:1067-1079.

• 18) S. Sonoda and M. Nishiguchi (2000). Delayed activation of post-transcriptional gene silencing and de novo transgene mthylation in plants with the coat protein gene of sweet potato feathery mottle potyvirus.Plant Sci. 156:137-144.

• 19) Y. Matsushita, K. Hanazawa, K. Yoshioka, T. Oguchi, S. Kawakami, Y. Watanabe, M. Nishiguchi and H. Nyunoya (2000). Characterization of a protein kinase that forms a stable complex with themovement protein of tomato mosaic tobamovirus J. Gen. Virol. 81:2095-2102.