分子生物資源学研究室

最近の研究論文

• 1)Haque, Y.,Tanaka, S.Sonoda.M.,Nishiguchi,M.(2006) Analysis of transitive RNA silencing after grafting in transgenic plants with the coat protein gene of Sweet potato feathery mottle virus. Plant Mol. Biol. (in press)

• 2) M. Nishiguchi, S. Yamasaki, X.-Z. Lu, A. Shimoyama, K. Hanada, S. Sonoda, M. Shimono, J. Sakai, Y. Mikoshiba, and I. Fujisawa (2006) Konjak mosaic virus: the complete nucleotide sequence of the genomic RNA　and its comparison with other potyviruses. Arch. Virol.151:1643-1650.

• 3) M. Asano, R. Satoh, A. Mochizuki, S. Tsuda, T. Yamanaka, M. Nishiguchi, K. Hirai, T. Meshi, S. Naito, M. Ishikawa (2005) Tobamovirus-resistant tobacco generated by RNA interference directed against host genes. FEBS Lett. 579(20):4479-4484.

• 4) M. Shimono, M. Ino, S. Sonoda, T. Fujimura and M. Nishiguchi (2005) Inverse-corelation between the Virus resistance in Nicotiana benthamiana plants showing different levels of GFP gene silencing. J. Gen. Plant Pathol. 71: 147-152.

• 5) S. Sonoda, M. Nishiguchi and H. Tsumuki (2005) Evaluation of virus resistance conferred by the NS gene sequences from Tomato spotted wilt virus in transgneic plants. Breeding Sci. 55: 27-33.

• 6) Y. Matsushita, M. Ohshima, K. Yoshioka, M. Nishiguchi and H. Nyunoya (2003). The catalytic subunit of protein kinase CK2 phosphorylates in vitro the movement protein of Tomato mosaic virus. J Gen Virol 2003;84: 497-505

• 7) M. Shimono, J. Yazaki, K. Nakamura, N. Kishimot, S. Kikuchi,M. Iwano, K. Yamamoto,T. Sasaki, and M. Nishiguchi (2003). cDNA microarray analysis of gene expression in rice plants treated with probenazole, a chemical inducer of disease resistance.　J. Gen. Plant Pathol. 69: 76-82.

• 8) Y. Okada, A. Saito, M. Nishiguchi, T. Kimura, M. Mori, Y. Matsuda and T. Murata(2002). Microprojectile bonbardment-mediated transformation of sweet potato (Ipomoea batatas (L.) Lam). SABRAO J. Breed. Genet. 34(1), 1-8.

• 9) Y. Okada, M. Nishiguchi, A. Saito, T. Kimura, M. Mori, K. Hanada, J. Sakai, Y. Matsuda and T. Murata(2002). Inheritance and stability of the virus-resistance gene in the progeny of　transgenic sweet potato. Plant Breed. 121, 249-253.

• 10) Y. Matsushita, O. Miyakawa, M. Deguchi, M., M. Nishiguchi1 and H. Nyunoya (2002). Cloning of a tobacco cDNA coding for a putative transcriptional coactivator MBF1 that interacts with the tomoto mosaic virus movemnet protein. J. Expt. Bot. 53:1531-1532.

• 11) Y. Okada, A. Saito, M. Nishiguchi, T. Kimura, M. Mori, K. Hanada, J. Sakai, C. Miyazaki, Y. Matsuda and T. Murata (2001). Virus resistance in transgenic sweet potato (Ipomoea batatas L.(Lam)) expressing the coat protein gene of sweet potato feathery mottle virus. Theor. Appl. Genet. 103: 743-751.

• 12) R. Nagata, M. Mori, K. Hanada and M. Nishiguchi(2001). An improved methodof reverse transcription and polymarase reaction (RT-PCR) to efficiently detect potyvirus, sweet potato feathery mottle virus (SPFMV) RNA in sweet potato. J. Gen. Plant Pathol. 67:164-168.

• 13) Y. Matsushita, M. Deguchi, M. Youda, M. Nishiguchi and H. Nyunoya(2001). The tomato mosaic tobamovirus movement protein interacts with a putative transcriptional coactivator KELP. Mol. Cells 12: 57-66.

• 14) N. Kubo, M. Takano, M. Nishiguchi and K.Kadowaki (2001). Mitochondrial sequence migrated downstream to a nuclear V-ATPase B gene is transcribed, but not functional. Gene 271;193-201.

• 15) S. Sonoda and M. Nishiguchi (2000). Graft-transmission of posttranscriptional gene silencing in transgenic plants; target specificity for RNA degradation is transmissible between silenced and non-silenced plants, but not between silenced plants. Plant J. 21(1):1-8.

• 16) S. Sonoda, M. Kanda, H. Koiwa, H. Kato and M. Nishiguchi (2000). The helper component-proteinase of sweet potato feathery mottle potyvirus facilitates systemic spread of unlelated viruses in Ipomoea nil. 　Phytopathology 90:944-950.

• 17) T. Sian-Hee, M. Nishiguchi, M. Murata and F. Motoyoshi (2000). The genome structure of kyuri green mottle mosaic virus in comparison with cucumber green mottle mosai virus. Arch. Virol. 145:1067-1079.

• 18) S. Sonoda and M. Nishiguchi (2000). Delayed activation of post-transcriptional gene silencing and de novo transgene mthylation in plants with the coat protein gene of sweet potato feathery mottle potyvirus.Plant Sci. 156:137-144.

• 19) Y. Matsushita, K. Hanazawa, K. Yoshioka, T. Oguchi, S. Kawakami, Y. Watanabe, M. Nishiguchi and H. Nyunoya (2000). Characterization of a protein kinase that forms a stable complex with themovement protein of tomato mosaic tobamovirus J. Gen. Virol. 81:2095-2102.

最近の著書・総説・その他

• 1) 西口正通　(2004)　２３．作物のストレス耐性-生物環境ストレス-ウイルス.　 新編農学大事典、養賢堂　p828-831.

• 2) 西口正通・霜野真幸　(2003)　プロベナゾール処理により発現の変化するイネ遺伝子群；マイクロアレイによる解析. 今月の農業　47(10):30-42.

• 3)西口正通・園田昌司・霜野真幸・田中良和　(2002)　ジーンサイレンシングとウイルス　植物ー微生物相互作用研究の現状と将来展望　植物感染生理談話会論文集 （第38号）ISSN　1345-8086：1-12.

• 4) M. Nishiguchi, Y. Okada, S. Sonoda, M. Mori, T. Kimura, K. Hanada, J. Sakai, T. Murata, Y. Matsuda, H. Fukuoka, T. Miyazaki, M. Nakano, T. Usugi and A. Saito (2001).　Sweetpotato feathery mottle virus derived resistance: CP mediated resistance and gene silencing. In Proceedings of International Workshop on Sweetpotato Cultivar Decline Study　(ed by Kyushu Natl Agric. Expt. Sta.). p120-124

• 5)西口正通・園田昌司・田中良和・霜野真幸 (2000)　植物におけるジーンサイレンシングとウイルス. ウイルス50(2):243-250.

• 6) M. Nishiguchi and F. Motoyoshi (1999) Transport of tobacco mosaic virus from cell-to-cell. In Tobacco Mosaic Virus, One Hundred Years of Contributions to Virology (eds. by K-B. G. Scholthof, J.G. Show and M. Zaitlin), p201ー202, APS Press.

生物生産コースのホームページに戻る