愛媛大学大学院農学研究科　生命機能学専攻　応用生命化学コース
Laboratory of Molecular Physiology and Genetics, Graduate School of Agriculture, Ehime University
愛媛大学農学部　生命機能学科　応用生命化学コース

原著論文

Saeki N, Yamamoto C, Eguchi Y, Sekito T, Shigenobu S, Yoshimura M, Yashiroda Y, Boone C, Moriya H. Overexpression profiling reveals cellular requirements in the context of genetic backgrounds and environments. PLoS Genet. 19: e1010732 (2023)

Sartiva H, Nishiwaki H, Akiyama K, Yamauchi S. Regiospecific and Enantiospecific Effects of the β-Benzyl-α-benzylidene-γ-butyrolactone Structure on Phytotoxic Fungi. J Agric Food Chem. 71: 6738-6746 (2023)

Ohnishi S*, Kawano-Kawada M*, Yamamoto Y, Akiyama K, Sekito T;　A vacuolar membrane protein Vsb1p contributes to the vacuolar compartmentalization of basic amino acids in Schizosaccharomyces pombe https://doi.org/10.1093/bbb/zbac041　Biosci. Biotechnol. Biochem, vol. 86 (6): 763-769 (2022)

Kawano-Kawada M, Ichimura H, Ohnishi S, Yamamoto Y, Kawasaki Y, Sekito T; Ygr125w/Vsb1-dependent accumulation of basic amino acids into vacuoles of Saccharomyces cerevisiae. Biosci. Biotechnol. Biochem, vol.85(5):1157-1164 (2021)

Sato A, Kimura T, Hondo K, Kawano-Kawada M, Sekito T; The vacuolar amino acid transport system is a novel, direct target of GATA transcription factors. Biosci. Biotechnol. Biochem, vol. 85(3):587-599 (2021)

Kawano-Kawada M, Ueda T, Mori H, Ichimura H, Takegawa K, Sekito T; Stm1 is a vacuolar PQ-loop protein involved in the transport of basic amino acids in Schizosaccharomyces pombe. BBA - Biomembranes, vol.1863(2):183507 (2021)

Kawano-Kawada M, Manabe K, Ichimura H, Kimura T, Harada Y, Ikeda K, Tanaka S, Kakinuma Y, Sekito T; A PQ-loop protein Ypq2 is involved in the exchange of arginine and histidine across the vacuolar membrane of Saccharomyces cerevisiae. Scientific Reports, vol.9 (1):15018 (2019)

Hidayati DN, Untari T, Wibowo MH, Akiyama K, Asmara W; Cloning and sequencing gB, gD, and gM genes to perform the genetic variability of bovine herpesvirus-1 from Indonesia. Veterinary World vol. 11(9):1255-1261(2018)

Pongcharoen P, Kawano-Kawada M; Identification and characterization of Candida tropicalis isolated from soil of sugarcane field in Thailand for ethanol production. Asia-Pacific Journal of Science and Technology, vol.23 (03) APST-23-03-01 (2018)

Fujiki Y, Teshima H, Kashiwao S, Kawano-Kawada M, Ohsumi Y, Kakinuma Y, Sekito T; Functional identification of AtAVT3, a family of vacuolar amino acid transporters, in Arabidopsis. FEBS Lett. vol. 591 (1): 5-15 (2017)

Yamauchi S, Wukirsari T, Ochi Y, Nishiwaki H, Nishi K, Sugahara T, Akiyama K, Kishida T; Syntheses of cytotoxic novel arctigenin derivatives bearing halogen and alkyl groups on aromatic rings. Bioorg Med Chem Lett. vol. 27(17):4199-4203 (2017)

Nishiwaki H, Nakazaki S, Akiyama K, Yamauchi S; Structure-Antifungal Activity Relationship of Fluorinated Dihydroguaiaretic Acid Derivatives and Preventive Activity against Alternaria alternata Japanese Pear Pathotype. J Agric Food Chem vol. 65(31):6701-6707(2017)

Kawano-Kawada M, Chardwiriyapreecha S, Manabe K, Sekito T, Akiyama K, Takegawa K, Kakinuma Y; The amino-terminal hydrophilic region of the vacuolar transporter Avt3p is dispensable for the vacuolar amino acid compartmentalization of Schizosaccharomyces pombe. Biosci. Biotechnol. Biochem. vol. 80 (12): 2291-2297 (2016)

Manabe K, Kawano-Kawada M, Ikeda K, Sekito T, Kakinuma Y. Ypq3p-dependent histidine uptake by the vacuolar membrane vesicles of Saccharomyces cerevisiae. Biosci. Biotechnol. Biochem, vol. 80 (6):1125-1130 (2016)

Kawano-Kawada M, Pongcharoen P, Kawahara R, Yasuda M, Yamasaki T, Akiyama K, Sekito T, Kakinuma Y; Vba4p, a vacuolar membrane protein, is involved in the drug resistance and vacuolar morphology of Saccharomyces cerevisiae. Biosci. Biotechnol. Biochem., vol. 80(2):279-287 (2016)

Chardwiriyapreecha S, Manabe K, Iwaki T, Kawano-Kawada M, Sekito T, Lunprom S, Akiyama K, Takegawa K, Kakinuma Y; Functional expression and characterization of Schizosaccharomyces pombe Avt3p as a vacuolar amino acid exporter in Saccharomyces cerevisiae. PLoS ONE, vol. 10: e0130542 (2015)

Lunprom S, Pongcharoen P, Sekito T, Kawano-Kawada M, Kakinuma Y, Akiyama K.Characterization of vacuolar amino acid transporter from Fusarium oxysporum in Saccharomyces cerevisiae. Biosci Biotechnol Biochem. vol. 79(12):1972-1979 (2015)

Tone J, Yamanaka A, Manabe K, Murao N, Kawano-Kawada M, Sekito T, Kakinuma Y : A vacuolar membrane protein Avt7p is involved in transport of amino acid and spore formation in Saccharomyces cerevisiae. Biosci. Biotechnol. Biochem. vol. 79(2):190-195. (2015)

Tone J, Yoshimura A, Manabe K, Murao N, Sekito T, Kawano-Kawada M, Kakinuma Y : Characterization of Avt1p as a vacuolar proton/amino acid antiporter in Saccharomyces cerevisiae. Biosci. Biotechnol. Biochem, vol. 79(5): 782-789 (2015)

Sekito T, Sugimoto N, Ishimoto M, Kawano-Kawada M, Akiyama K, Nishimoto S, Sugahara T, Kakinuma Y : Tributyltin induces cell cycle arrest at G1 phase in the yeast Saccharomyces cerevisiae. J. Toxicol. Sci. 39(2):311-317. (2014)

Sekito T, Chardwiriyapreecha S, Sugimoto N, Ishimoto M, Kawano-Kawada M, Kakinuma Y : Vacuolar transporter Avt4 is involved in excretion of basic amino acids from the vacuoles of Saccharomyces cerevisiae. Biosci Biotechnol Biochem. 78(6):969-975. (2014)

Sekito T, Nakamura K, Manabe K, Tone J, Sato Y, Murao N, Kawano-Kawada M, Kakinuma Y : Loss of ATP-dependent lysine uptake in the vacuolar membrane vesicles of Saccharomyces cerevisiae ypq1∆ mutant. Biosci. Biotechnol. Biochem. 78(7):1199-1202. (2014)

Pongcharoen P, Kawano-Kawada M, Iwaki T, Sugimoto N, Sekito T, Akiyama K, Takegawa K, Kakinuma Y.: Functional Expression of Schizosaccharomyces pombe Vba2p in Vacuolar Membrane of Saccharomyces cerevisiae. Biosci Biotechnol Biochem.77(9):1988-1990. (2013)

Shimazu, M., Itaya, T., Pongcharoen, P., Sekito, T., Kawano-Kawada, M., Kakinuma, Y : Vba5p, a novel plasma membrane protein involved in amino acid uptake and drug sensitivity in Saccharomyces cerevisiae. Biosci. Biotechnol. Biochem. 76(10):1993-1995　(2012)

Ishimoto, M., Sugimoto, N., Sekito, T., Kawano-Kawada, M., and Kakinuma, Y. : ATP-dependent export of neutral amino acids by vacuolar membrane vesicles of Saccharomyces cerevisiae. Biosci. Biotechnol. Biochem. 76(9):1802-1804.(2012)

Sugimoto, N., Iwaki, T., Chardwiriyapreecha, S., Shimazu, M., Kawano, M., Sekito, T., Takegawa, K., and Kakinuma, Y. Atg22p, a vacuolar membrane protein involved in the amino acid compartmentalization of Schizosaccharomyces pombe.　Biosci. Biotechnol. Biochem. 75:385-387 (2011)

Akiyama, K., Iwaki, T., Sugimoto, N., Chardwiriyapreecha, S., Kawano, M., Nishimoto, S., Sugahara, T., Sekito, T., and Kakinuma, Y. Bfr1p is responsible for tributyltin resistance in Schizosaccharomyces pombe. J. Toxicol. Sci. 36:117-120 (2011)

総説
関藤 孝之, 河田(河野)美幸; 1. 液胞／リソソームアミノ酸トランスポーターによる細胞内アミノ酸ホメオスタシス. 生化学, vol. 95, 747-756 (2023)

Kawano-Kawada M, Kakinuma Y, Sekito T; Transport of Amino Acids across the Vacuolar Membrane of Yeast: Its Mechanism and Physiological Role. Biol Pharm Bull. vol. 41(10):1496-1501 (2018)

関藤 孝之、柿沼 喜己; 液胞／リソソーム膜を介したアミノ酸輸送の分子機構.
化学と生物, vol. 54, 324-334 (2016)

Sekito T., Fujiki Y., Ohsumi Y. and Kakinuma Y; Novel families of vacuolar amino acid transporters. IUBMB Life, vol. 60, 519-525 (2008)

Kamada Y., Sekito T. and Ohsumi Y. Autophagy in yeast: a TOR-mediated response to nutrient starvation. Curr. Top. Microbiol. Immunol., 279:73-84 (2004)

中戸川 仁、花田 孝雄、鎌田 芳彰、小原 圭介、関藤 孝之（2006）オートファゴソームを紡ぎ出す5つのAtgタンパク質群. 蛋白質核酸酵素 ８月増刊号:1457-1463.

関藤 孝之、大隅 良典（2003）　酵母の飢餓応答-オートファジー研究の進展.　清酒酵母の研究～９０年代の研究～ 46-49.　

著書
関藤孝之「オートファゴソームの内膜分解と分解産物の再利用に機能する分子装置」.
オートファジー　分子メカニズムの理解から病態の解明まで. 大隅良典 監修／吉森保・水島昇・中戸川仁 編集. 南山堂. p61-70. 2018年.