Work of Hiroshi Nonami with a pressure probe. (Plant Physiology (1993) 102: 13-19.)
Plant Biophysics/Biochemistry Research Laboratory studies physiological responses in crop plants under environmental stresses caused by changes in nutrient, temperature, light and moisture conditions. The major research projects are related to plant water relations. Also, hydroponic culture and nutritional absorption mechanisms are studied. Recently, mass spectrometry (MALDI TOF-MS and ESI TOF-MS) is introduced, and microanalyses for proteins, oligosaccharides, nucleic acids (DNA/RNA) and polymers in plant cells are studied. The joint works with Dr. Koichi Tanaka, the Nobel Prize Laureate in Chemistry 2002, developed effective laser-ionization matrices for high molecular weight molecules (see Nonami’s Web page for details).
Psychrometers, pressure chambers and pressure probes are used to measure the water status of plants, i.e., water potential, osmotic potential and turgor.
Pressure probes allow us to extract cell solution in intact plants as shown in the top photo, and combinations of pressure probe and MALDI mass spectrometry techniques can be used for studying molecular biology on the level of cells in intact plants.
Regarding mass spectrometry research, we have been cooperating with Prof. R. Erra-Balsells of the University of Buenos Aires, which has the academic cooperation agreement with Ehime University. Prof. Dr. Erra-Balsells specializes in mass spectrometry, photochemistry and sugar chemistry. She visits this laboratory for 4-6 months every year, and teaches basic chemistry and mass spectrometry techniques for graduate research study. Additionally, other professors from the University of Buenos Aires sometimes come to our lab for research collaboration. After finishing the doctorate study from our lab, chances of postdoctral studies increase in Europe, the United States of America and elsewhere. Particularly, continuation of postdoctral study in our lab funded by JSPS is welcome (2-year program). The University of Buenos Aires has produced 3 Nobel Prize Laureates in Physiology/Medicine and Chemistry related to sugar chemistry and sugar metabolisms.
Academic Agreement between Ehime University and the University of Buenos Aires (Spanish version)Grant-in-Aid (S) (FY2008-2012): Cell Turgor Measurement - Probe Electrospray Ionization (PESI) Mass Spectrometry for Molecular Profiling Techniques
Grant-in-Aid (S) (FY2012-2016): “Speaking Cell Approach” by on-site/real-time cellular and molecular measurementsCorresponding Address:
Prof. Dr. Hiroshi Nonami
College of Agriculture, Ehime University
Tarumi, Matsuyama, 790-8566 Japan
E-mail: nonami@agr.ehime-u.ac.jp
Phone/Fax: +81-89-946-9824
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